Phospho-p38 mapk thr180/tyr182
WebPhospho-p38 MAP Kinase (Thr180/Tyr182) (12F8) Rabbit mAb detects endogenous levels of p38 MAPK only when dually phosphorylated at threonine 180 and tyrosine182. It will also react with p38 singly phosphorlyated at Thr180. This antibody does not cross-react with … Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when activated by phosphorylation at threonine 180 and tyrosine 182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182.
Phospho-p38 mapk thr180/tyr182
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WebThe NeuroMab™ anti-Phospho-p38 MAPK (Thr180/Tyr182) antibody detects endogenous levels of p38 MAPK only when dually phosphorylated at threonine 180 and tyrosine182. It will also react with p38 singly phosphorlyated at Thr180. This antibody does not cross … WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Validations Biological Strategies Applications Flow cytometry/Cell sorting (FC/FACS) Immunofluorescence (IF) Western Blotting (WB) Reactivity Chlorocebus sabaeus (Green …
Web28796-1-AP targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, ELISA applications and shows reactivity with Human samples. Background Information A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. WebThe cells were labeled with Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal Antibody (Product # 36-8500) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary …
Webfor Phospho (Thr180/Tyr182) and Total p38 levels by InstantOne ELISA™. Product Information . Contents: p38 MAPK (Total/Phospho) InstantOne ELISA™ Catalog Number: 85-86023. Temperature Limitation: Store at 2 -8°C. Batch Code: Refer to vial Use By: Refer … WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, …
WebThe recovered lysate was incubated for 30 minutes on ice and centrifuged at 17,800 xg for 15 minutes. The supernatant was then analyzed by Western blotting with specific antibodies for phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204), phospho-p38 MAPK (Thr180/Tyr182), phospho-NF-κB p65 (Ser536), and GAPDH as previously described. 8,9. Statistical ...
WebMar 3, 2024 · It has been previously reported that p38 MAPK signaling is activated downstream of Syk in PMNs . We observed that fMLF-mediated activation of p38 MAPK at Thr180 and Tyr182 in human PMN is significantly decreased by sialidase inhibitors (Sia … gps will be named and shamedWebPhospho-p38 MAP Kinase (T180/Y182) Cell-Based ELISA Summary. Assay Type. Solid Phase Sandwich ELISA. ... p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the … gps west marineWebfor Phospho (Thr180/Tyr182) and Total p38 levels by InstantOne ELISA™. Product Information . Contents: p38 MAPK (Total/Phospho) InstantOne ELISA™ Catalog Number: 85-86023. Temperature Limitation: Store at 2 -8°C. Batch Code: Refer to vial Use By: Refer to vial. Description gps winceWebHuman p38 MAPK peptide phosphorylated at Thr 180 and Tyr182 Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/ml Storage & Handling The … gps weather mapWebThis activation results in the phosphorylation of residues Thr180 and Tyr182. This antibody is specific for the 39 kD p-38 protein phosphorylated at Thr180/Tyr182 It is purified by sequential chromatography on protein A and phospho- and dephospho- peptide affinity columns. Product Details Technical data sheet Product Details Verified Reactivity gpswillyWebIn the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 … gps w farming simulator 22 link w opisieWebApr 14, 2024 · Caffeine activates p38 MAPK, the human homolog to the Hog1 protein that orchestrates the high-osmolarity glycerol (HOG) response to osmotic stress in the yeast Saccharomyces cerevisiae. ... The upper blot shows the Western signal with the dually phosphorylated Hog1 detected using anti-phospho p38 (Thr180/Tyr182) monoclonal … gps wilhelmshaven duales studium