WebApr 26, 2016 · Myostatin (MSTN) is a negative regulator of muscle mass, related to muscle growth and differentiation. ... Crispo, M. et al. Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 ... WebFeb 25, 2024 · Generation of myostatin-knockout chickens mediated by D10A-Cas9 nickase 1 INTRODUCTION. Quantitative genetics and genomic selection are conventionally …
Site-Directed Genome Knockout in Chicken Cell Line and Embryos ... - PubMed
WebDec 26, 2024 · In mammals, Myostatin (MSTN) is a known negative regulator of muscle growth and development, but its role in birds is poorly understood. To investigate the molecular mechanism of MSTN on muscle growth and development in chickens, we knocked out MSTN in chicken fetal myoblasts (CFMs) and sequenced the mRNA … WebKnockout of chicken myostatin ( MSTN) gene and identification of mutant genotype in the targeted sites. (A) Fluorescence-activated cell sorting (FACS) of GFP-positive cells after co-transfection of the Cas9-D10A nickase expression vector with green fluorescent gene ( GFP) gene and targeted multiplex guide RNAs (gRNAs). blank w9 printable form
Generation of myostatin edited horse embryos using CRISPR/Cas9 …
WebJan 10, 2024 · Three sgRNAs used to knockout the STRA8 gene in DF-1 cells, and chicken ESCs were created. The Cas9/sgRNA plasmid was introduced into cells using the lipofection method. The efficiency of knockout in DF-1 cells and ESCs was 25% and 23%, respectively. In this study, PEI was also used to introduce the Cas9/gRNA plasmid into chicken embryos. WebAug 1, 2024 · Opposite to quail, body weight of the male chicken is greater than the female chicken and feed efficiency of the male is better than the female as well (Benyi et al., 2015), ... Generation of myostatin-knockout chickens mediated by D10A-Cas9 nickase. FASEB J, 34 (2024), pp. 5688-5696. CrossRef View in Scopus Google Scholar. Lee et al., 2024. WebDec 26, 2024 · In mammals, Myostatin (MSTN) is a known negative regulator of muscle growth and development, but its role in birds is poorly understood. To investigate the … blank w 9 printable form template