His6标签抗体
Webb5 apr. 2005 · The presence of a His6 tag enables the isolation of peptide or protein products directly from ligation reaction mixtures by Ni-NTA affinity column purification. … Webb6×His Tag多肽（6×His Tag Peptide）是由6个组氨酸残基组成的多肽，组氨酸残基侧链对镍离子具有高选择性亲和力。在免疫分析实验中，6×His Tag多肽与6×His融合蛋白竞争性 …
His6标签抗体
Did you know?
WebbAnti-His 6 is a monoclonal antibody to His 6 -tagged proteins. Anti-His 6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence. Webb일반 설명 N-terminal His6-tagged, recombinant, human Hck, amino acids 230-497 Product Source: Expressed by baculovirus in Sf21 insect cells 포장 10 , 250 μg Also available in 250 µg size --call for pricing and availability and reference catalog number 14-577M when ordering the 250 µg size. 애플리케이션 Research Category Inflammation & Immunology …
WebbHis标签是蛋白质重组技术中经常用到的一种标签，其序列为六个组氨酸HHHHHH， 其特点是分子量小，基本不改变蛋白质的生物结构，不改变蛋白质的溶解性，更重要的是它使 … Webb28 apr. 2024 · His-tag 蛋白質純化產品特輯. 在使用原核細胞（如大腸桿菌）或真核細胞（如酵母菌、昆蟲細胞或哺乳動物細胞）大量表現重組蛋白質時，通常會在目標蛋白基 …
Webb22 juni 2024 · 载体蛋白His6-H4-NLS的制备方法，包括以下步骤： 1）将pET28a-His6-H4-NLS质粒导入到原核表达菌BL21（ DE3 ）中，挑单菌落，接种到5mlLB培养基，37℃震荡培养10小时，1/100接菌至1000mlLB培养基中，继续37℃震荡培养2.5-3.5小时，至OD600至0.8-1.0，加入终浓度0.1mMIPTG，37℃震荡培养4-5小时； 2）4℃4000g离 … Webb22 mars 2005 · Synthetic strategies ( A, conventional; B, His 6 tag-assisted) for the consecutive chemical ligation of several peptide segments to give a target protein/polypeptide chain. His 6 tag-assisted chemical protein synthesis dramatically speeds up and facilitates the isolation and handling of intermediate ligation products ( B …
Webb22 okt. 2006 · 6组氨酸标签与目的蛋白的基因之间构建酶切位点，比如凝血酶的酶切位点，在用Ni-NTA树脂螯合目的蛋白质后不用咪唑洗，而直接用凝血酶（thrombin）加缓冲 …
WebbHis-Tag Antibody (H-3) is an IgG 1 κ mouse monoclonal His-Tag antibody (also designated 6X His-Tag antibody or His-Probe antibody) suitable for the detection of the His-Tag … scale of injusticeWebbHis标签蛋白快速检测试剂盒. His标签是由6个组氨酸（His-His-His-His-His-His）组成的短肽，通常连接在蛋白质的N端或C端，能够用于重组蛋白的分离纯化。. His标签的分子量 … saxcat\u0027s gaming cornerWebbOur 6x His Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Goat, Sheep and Chicken. … saxbys west chester paWebb15 juni 2016 · 豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座,心理学等数亿实用 ... saxbys west chester applicationWebbOne way around this problem is to fuse an aggregation-prone protein to a highly soluble partner. E. coli maltose-binding protein (MBP) is widely recognized as a premier solubilizing agent. In this chapter, we describe how to construct dual His6-MBP-tagged fusion proteins by Gateway (®) recombinational cloning and how to predict their yield … scale of inquiry geographyWebb11 apr. 2024 · Cell Host Microbe. 12, 20-33 (2012) (PMID: 22817985) Ma L et al. Serum anti-CCNY autoantibody is an independent prognosis indicator for postoperative patients with early-stage nonsmall-cell lung carcinoma. Dis Markers. 35, 317-325 (2013) (PMID: 24167380) ※在庫につきましては2024年04月11日 17時00分時点における在庫数を ... saxbys university city hoursWebb21 aug. 2024 · Plasmid pET28a_His-TEV-EiCsm6 from Dr. Jennifer Doudna's lab contains the insert Enterococcus italicus Csm6 (codon optimized) and is published in Nat Chem Biol. 2024 Sep;17(9):982-988. doi: 10.1038/s41589-021-00842-2. Epub 2024 Aug 5. This plasmid is available through Addgene. scale of inquiry meaning